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Technical characteristics of the real-time PCR machine "ANK"
Machines ANK are characterized by:
- DNA/RNA quantitation from a single to 109 copies
- up to five-colours fluorescence detection, possibility of detecting a great number of fluorescent dyes - SYBR Green I, FAM, R 6 G, HEX, JOE, ROX, TAMRA, Cy 5 and others
- possibility of multiplex PCR design
- high sensitivity of all detection channels, which allows working with down to 0,5 pico-mole probes per reaction
- user-friendly, convenient software, modern algorithms of threshold cycles calculation and ability to choose variants of data analysis
- reduction of contamination risk, time saving, simplification of PCR-laboratory organizationè
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Characteristics | ÀÍÊ-4 | ÀÍÊ-16 | ÀÍÊ-32 | ÀÍÊ-64 |
Number of samples | 4 | 16 | 32 | 64 |
Type and volume of test tubes, ml | Standart,with transparent dome lid; 0,2 |
Temperature range, °Ñ | 4 – 99 |
Temperature range, °Ñ | ± 0.15 |
Temperature precision in range from 40 tî 95°Ñ, °Ñ | ± 0.1 |
Heating and cooling rate of tfe thermal block °Ñ per second | 2,5 | 1.5 |
Adjustable heating of the thermal lid,°Ñ | 95–110 |
Accuracy of maintaining lid temperature ,°Ñ | ± 1 |
Optical unit |
Light sourse | 4 LED’s | Halogen lamp |
Detector | Photo-electronic mdltiplier tube |
Consumption wavelengthes, nm | 490, 530, 580, 630 |
Registrarion wavelengthes, nm | 520, 550, 610, 670 |
Sensitivity of each channel (FAM,R6G,ROX è Cy5) not more then, Ì | 10-9 | 2.5·10-9 |
General characteristics |
Power consumption, W | 100 | 300 |
Size, mm | 320 õ 259 õ 220 | 340x400x420 |
Weight, kg | 5,5 | 26 |
Warranty | 2 year |
Analytical characteristics of real-time PCR machine ANK |
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Possibility of identifying small differences in amounts of original DNA | Wide dynamic range of DNA quantitation |
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High accuaracy and fine precision of measurement results. The spead in values of threshold cyccles for 32 equal samples amounts to ± 0.11 | Possibility of multiplex PCR design |
Developer
Institute for analytical instrumentation Russian Academy of Sciences | |